Differential centrifugation, a method intro­duced to cellular study in the early forties because of the noted biologist and Nobel laureate Albert Claude, has become the classic procedures for isolating subcellular particles and entails the stepwise elimination of courses of particles by successive centrifugations at increas­ing RCF.

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As being the smaller sized particles are sedimented, they subsequently is going to be contaminated by even smaller particles. Actually, the one particles obtained in rather pure type will be people who sediment most slowly and gradually.

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The RCF or “g force” applied to particles in the course of cen­trifugation may well easily be calculated applying equation 12-four and is impartial of the Bodily properties on the particles being sedimented.

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